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Carcass examinations in the Atlantic Unusual Mortality Event during winter 2021-2022

Updated April 6, 2022

Background

dead manatee on floor with a flat body

Emaciated carcass. The body is flattened.

An Unusual Mortality Event (UME) caused by starvation due to lack of forage in the Indian River Lagoon (IRL) started in December 2020 and is ongoing on the Atlantic coast. The IRL provides vital habitat for manatees in all seasons and is central in manatee migration on this coast. Consequently, the effects of malnutrition have been documented in manatees all along the Atlantic coast. Mortality has been high during last winter (2020-2021) and this winter because colder temperatures add extra health stressors to manatees that are already compromised by chronic malnutrition. The negative health impacts of malnutrition are long-lasting and have been documented in Atlantic coast carcasses during the warmer months as well.

Necropsy findings of chronic starvation in manatee UME carcasses

External features of emaciation range from flattening of the body, loose skin, folds in the belly, distinction of head from the rest of the body with a dip in the neckline (‘peanut-head’), to visible outline of skeletal structures. On necropsy, the transformation of fat, muscle and connective tissue into a watery gelatinous substance (‘serous atrophy’) suggests the manatees in this UME experienced prolonged starvation and malnutrition, depleting their bodies. Lower than normal liver weights are consistent with chronic starvation. This state of extreme wasting is further evidenced by atrophy of vital organs such as liver, heart, and diaphragm on microscopic examination.

three images of a dead manatee, fat and muscle tissue samples, and purple histology slide

(A) Emaciated carcass. There is a distinct dip in the neck region (‘peanut-head’) and sunken eyes; (B) Tissue section of fat and muscle from two emaciated manatees. The manatee on the left died from cold stress disease and still had grossly normal fat despite it being thin and depleted. The fat and muscle of the manatee on the right that died from primary starvation was depleted but also very watery (‘serous atrophy’), further emphasizing the prolonged state of wasting; (C) Microscopic view of liver atrophy. The liver cells (light pink with purple nucleus) are smaller than normal. The brown pigment in the cells is from the breakdown of the components that make up the cell. Image credit Dr. Dave Rotstein.

Carcass sampling

lab counters with dozens of glass jars

Histopathology sample collection has expanded to meet the needs of specific health investigations. Before this UME, histopathology cases averaged about 30 cases per year.

All manatee carcasses, regardless of disposition, are documented in the manatee mortality database after verification by FWC manatee biologists. Manatee carcass numbers have increased over time (with added peaks during UMEs) and it is no longer feasible nor necessary to necropsy nearly every carcass in Florida. To meet essential information needs, necropsies are performed under surveillance protocols in order to track key threats and additionally certain carcasses are selected for more detailed necropsies and specific health investigations. These detailed necropsies are specifically needed for the investigation of starvation which requires extra data and analyses (e.g., weights, measurements, microscopic examination). For example, more than 100 carcasses have been sampled for investigation of tissue atrophy in this UME. This is done from formalin-fixed tissues, which are made into microscopic slides for examination by a veterinary pathologist (histopathology). In this UME, portions of a carcass may still be examined for findings consistent with starvation. Such carcasses are listed as ‘Verified; Not Necropsied’ because a full necropsy was not performed and a primary cause of death was not determined, but starvation findings are still reported in the mortality database to aid in documenting the extent of starvation among carcasses.

Recent starvation data (preliminary)

Between December 1, 2021, and March 31, 2022, 457 manatee deaths were verified on the Atlantic coast. Primary starvation is the leading cause of death in necropsied carcasses on the Atlantic coast (these deaths are included in the ‘Natural; Other’ category in FWRI mortality web tables). Out of the 313 carcasses in the ‘Verified; Not Necropsied’ category, 231 received a fat, muscle, and stomach inspection; 204 of these (88%) had findings consistent with malnutrition. Necropsy data indicate that manatees had experienced starvation and malnutrition before entering winter. Overall, the total number of verified carcasses on the Atlantic coast was lower than last winter when 582 carcasses were documented during this timeframe. This does not mean that manatee health improved from last winter. To the contrary, necropsies demonstrated severe, extensive tissue atrophy, which is likely the result of experiencing malnutrition through multiple seasons. It is possible that a relatively warm December and fewer winter cold fronts contributed to the lower total mortality number this winter compared to last. Carcass numbers remained unprecedented and far above what can be expected in normal winters on this coast. In addition, there is continued concern for the health of the manatees that survived this winter but will continue to experience shortages in proper nutrition.

bar chart showing number of manatee carcasses and cause of death

Carcass examinations show that starvation is the leading cause of death among fully necropsied carcasses. Findings of starvation are also present in the majority of carcasses that are verified but not fully necropsied (VNN in blue).